trypsin-like protein which momentarily appears immediately before DNA synthesis in the cell cycle of E coli synchronized by phosphate starvation & which is closely related to the initiation of DNA replication; hydrolyzes fluorogenic substrate for trypsin, but does not hydrolyze fluorogenic substrates for chymotrypsin & elastase; inhibited by leupeptin, antipain , 4-nitrophenyl 4-guanidionobenzoate and by various esters of trans-4-guanidinomethylcyclohexane carboxylic acid; MW 66 kDa
