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Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Entry Term(s)
Cytofluorometry, Flow
Cytometry, Flow
Flow Microfluorimetry
Fluorescence-Activated Cell Sorting
Microfluorometry, Flow
NLM Classification #
QY 95
Public MeSH Note
82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90
Online Note
use FLUOROIMMUNOASSAY to search FLOW MICROFLUORIMETRY 1989-90
History Note
82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.