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Microscopy, Fluorescence, Multiphoton MeSH Descriptor Data 2022


MeSH Heading
Microscopy, Fluorescence, Multiphoton
Tree Number(s)
E01.370.350.515.458.500
E01.370.350.515.717.250
E05.595.458.500
E05.595.717.250
Unique ID
D036641
RDF Unique Identifier
http://id.nlm.nih.gov/mesh/D036641
Scope Note
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
Entry Term(s)
Fluorescence Microscopy, Multiphoton
Multiphoton Excitation Microscopy
Multiphoton Fluorescence Microscopy
Previous Indexing
Microscopy, Confocal (1997-2002)
Microscopy, Fluorescence (1996-2002)
Public MeSH Note
2003
History Note
2003
Date Established
2003/01/01
Date of Entry
2002/07/03
Revision Date
2017/06/30
Microscopy, Fluorescence, Multiphoton Preferred
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