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Freeze Etching MeSH Descriptor Data 2025


MeSH Heading
Freeze Etching
Tree Number(s)
E01.370.225.500.620.620.260.400
E01.370.225.750.600.620.260.400
E05.200.500.620.620.260.400
E05.200.750.600.620.260.400
Unique ID
D005613
RDF Unique Identifier
http://id.nlm.nih.gov/mesh/D005613
Annotation
do not confuse with FREEZE FRACTURING (wherein a cryoprotectant is used)
Scope Note
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
Previous Indexing
Freezing (1966-1968)
Histological Techniques (1966-1968)
Microscopy, Electron (1966-1968)
Public MeSH Note
73
History Note
73(69)
Date Established
1973/01/01
Date of Entry
1999/01/01
Revision Date
2011/06/24
Freeze Etching Preferred
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