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Freeze Fracturing MeSH Descriptor Data 2022


MeSH Heading
Freeze Fracturing
Tree Number(s)
E01.370.225.500.620.620.260
E01.370.225.750.600.620.260
E05.200.500.620.620.260
E05.200.750.600.620.260
Unique ID
D005614
RDF Unique Identifier
http://id.nlm.nih.gov/mesh/D005614
Annotation
do not confuse with FREEZE ETCHING (wherein no cryoprotectant is used)
Scope Note
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
NLM Classification #
QY 95
Previous Indexing
Freeze Etching (1969-1974)
Public MeSH Note
75
History Note
75
Date Established
1975/09/01
Date of Entry
1974/11/11
Revision Date
2011/06/24
Freeze Fracturing Preferred
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