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Chromatin Immunoprecipitation MeSH Descriptor Data 2022


MeSH Heading
Chromatin Immunoprecipitation
Tree Number(s)
E05.393.170
E05.478.605.160
Unique ID
D047369
RDF Unique Identifier
http://id.nlm.nih.gov/mesh/D047369
Scope Note
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Entry Term(s)
Immunoprecipitation, Chromatin
Previous Indexing
Precipitin Tests (2000-2004)
Public MeSH Note
2005
History Note
2005
Date Established
2005/01/01
Date of Entry
2004/07/19
Chromatin Immunoprecipitation Preferred
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